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Hours of Operation/Sample Dropoff

Monday - Friday: 9am-5pm
or call for special arrangements.

We provide a sample drop off freezer if no one is available to take your samples. Instructions and a form are available in the Main lab.

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Overview of Microarray Choices

What are expression microarrays?
GeneChip expression microarrays enable researchers to simultaneously monitor genome-wide expression profiles. This global view of expression aids in the understanding of the biological mechanisms of complex diseases, and can lead to the identification of new drug targets in ways never before possible.

What services does the Facility offer?
The Core Facility offers the Affymetrix GeneChip platform to carry out expression studies. There are many choices when selecting this service, including the labeling method, the labeling chemistry, the array configuartion and the array type. Please review the following to familiarize yourself with this service, then select the appropriate service from the left side menu [3' or WT] for more details.

IMPORTANT

It is highly recommended and strongly encouraged for you to have a consultation with us prior to commencing a study. With ever-changing technology and constant improvements and updates to both labeling methods and arrays, we want to make sure you are selecting the best approach for your studies.
Contact Martina Veigl for a consult.

Microarry Options

       
Labeling Method
Robotic
OR Hand labeling

Robotic labeling is cheaper, however, a minimum of 16 samples must be labeled. Hand labeled samples can be delivered in tubes. Robotic labeled samples must be delivered in plates at a specific concentration.

Labeling Chemistry
OR

Nugen chemistry allows for the labeling of small amounts of RNA. The pricing is the same.

Array Configuration
Cartridge
OR Pegs

Cartridge arrays are individual arrays that hold 1 sample. Peg arrays contain multiple arrays on a plate in either 16, 24 or 96 formats. Peg arrays are cheaper than cartridge arrays but you must use them in the provided format options.

Array Type: [WT or 3']
Detection of splice variants.
3' interrogation

3' arrays have probes that interrogate the 3' end of the gene, detecting only 1 variant.

  OR    
Whole transcript [Exon or Gene arrays]

WT arrays have probes across the length of the gene and can detect multiple splice variants. WT arrays come as Exon or Gene arrays: the difference lies in the probe distribution.

WT Array Type: [Gene or Exon] - Probe Distribution.

Exon arrays utilize approximately 40 probes per gene. Gene arrays utilize approximately 26 probes across the length of the gene.