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Hours of Operation/Sample Dropoff

Monday - Friday: 9am-5pm
or call for special arrangements.

We provide a sample drop off freezer if no one is available to take your samples. Instructions and a form are available in the Main lab.

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Real Time PCR [TaqMan] - User Instructions

Sample Registration:

Please email completed forms to gegf.rtpcr@gmail.com

How to Bring Samples:

Samples may be brought as RNA or cDNA. RNA samples must be free of inhibitors of PCR and reverse transcription. Ideally, the concentration should be between 0.02 and 0.2 ug/ul in DEPC or RNase-free water, and samples should be 75ul or greater. However, high quality results can be obtained with less material. Unless you are using samples for an Endogenous Control Determination, it is not necessary to know the exact concentration of your samples. RNA should be delivered in 0.5 ml microfuge tubes with the sample number on the top of the tube.

IMPORTANT

The Facility will NOT quantitate your samples or measure the volume. We assume that samples will be delivered according to the criteria above. If they are not, we cannot guarantee that the assays will work. If your samples cannot meet these criteria, please contact a facility member to discuss how to deliver your samples.