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Monday - Friday: 9am-5pm
or call for special arrangements.

We provide a sample drop off freezer if no one is available to take your samples. Instructions and a form are available in the Main lab.

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3' Expression Microarrays - Technology

Basic Concepts:

GeneChip hybridization technology makes it possible to assess the relative mRNA expression levels of thousands of genes and ESTs simultaneously. Quantitative and reproducible detection of transcripts over a wide range of mRNA expression levels is possible. Target genes are probed by single stranded 25-mer oligonucleotides constructed on the arrays. Each gene is represented on the array by 11-20 different probe pairs. Each probe pair consists of a perfect match oligonucleotide and a single base mismatch oligonucleotide. The difference in hybridized signal between members of the probe pairs is used to assess expression levels and to ensure against non-specific hybridization. Probes are chosen from unique regions at the 3'-end of the gene, which allows for detection of individual transcripts within gene families.

Standard 3' Eukaryotic Gene Expression Assay:

The standard assay behind the use of GeneChip arrays for gene expression is simple: labeled cDNA or cRNA targets derived from the mRNA of an experimental sample are hybridized to nucleic acid probes attached to the solid support. By monitoring the amount of label associated with each DNA location, it is possible to infer the abundance of each mRNA species represented. Although hybridization has been used for decades to detect and quantify nucleic acids, the combination of the miniaturization of the technology and the large and growing amounts of sequence information, have enormously expanded the scale at which gene expression can be studied.